1. Summary of Technical Progress

1. Current Technical Status

2. Tasks of the work plan

• State / Situation at the beginning of the current quarter

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• Fulfilled work

 

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• Results by the end of the current quarter

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Biological samples were taken from birds belonging to 2 species (Table 1).  

Table 1 

The list of birds from which samples on influenza virus were taken in Quarter 7 (December 2010- February 2011)  

• Personnel Commitments

 

• State / Situation at the beginning of the current quarter

At the project beginning molecular genetic researches of birds have been conducted. Virological researches on cell culture of dog’s kidney was conducted, however, there was no virus isolation. There were samples with cytopathogenic effects.  

• Fulfilled work

 

  Total number of conducted researches is 343.  33 species of birds were exposed to molecular-genetic researches, presence of influenza A were detected in 3 bird species.    

• Results by the end of the current quarter

 

Table 2

Results of monthly molecular-genetic researches of birds

 

Positive samples in PCR reaction in a Real time mode with the threshold line before 30 cycles, thus indicating the presence of sufficient RNA RNase P gene indicating the specimen is of acceptable quality. At the same time primary infection and 2 passages on a cell culture have been conducted out of them in 3 cases (from materials from Night Heron and 2 Mallards ) cytopathogenic virus effect is detected. These isolates are typed in hemagglutination reaction and hemagglutination inhibition of reaction. Preliminary results of virological researches see below.  
 
 
 
 
 
 
 

Table 3

Results of virological researches

Total number of conducted researches is 307.  46 species of birds were exposed to molecular-genetic researches ( table 4). Research were conducted by real time  PCR on influenza A, subtypes Н1, Н3 and  Н5. During researches of 46 bird species in 2 samples (0,6%)  from 1 bird species  presence of influenza A virus is detected, however results of sybtyping on Н1, Н3. we find  on sample  Н5 were negative Positive samples on influenza A were frozen at -70 Celsius degree for further researches. It is necessary to conduct researches on subtypes H2, H4, H6, H15 and N1-N9 with foreign collaborators. For this purpose business trips to laboratories of foreign collaborators to exchange experiences is necessary. 

 

Results of molecular-genetic investigations of birds in Quarter 6 (September-December, 2010)

                                                                                                       Table 4

 

  
 
 
 
 

Out of 307 researches birds, 293 have been researched in Issyk-Kul oblast. In Issyk-Kul oblast positive samples of avian influenza were found.                                       

  Table 5

  

 During analysis of influenza A virus detection among waterfowls it was determined that more often in the reporting period the positive A influenza sample have been detected among Mallards (2 positive).

       

Detection of influenza virus among various species of waterfowls in Quarter 6 (September-December, 2010)

                                                                                                                                                 Table 6